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Antibody to HLA, or the Fab fragment, or the F(ab)2 fragment for 1 h, and the amount of released VWF was measured (n 3 SD). (B) Cross-linked Fab fragment activates exocytosis. HAEC were incubated with the Fab fragment of antibody to HLA, and then mouse F(ab)2 antibody to human Fab was added for 1 h to cross-link Fab monomers (n 3 SD; *, P 0.05 vs. control).Fig. 6. Antibody activates leukocyte adh
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Cterize the infection over the life cycle of L. salmonis. In Trials 1?, the mean seawater temperature was 10.5, 11.5 and 8.5 , respectively, reflecting ambient conditions in early November (Trials 1 and 2) and from mid-January to late February(Trial 3). All fish were acclimated to full-strength seawater a minimum of 10 days prior to exposure to L. salmonis (see Additional file 2: Table S1 for fis
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Control testing were screened for respiratory viruses using previously optimized and described PCR and reverse transcriptase PCR assays. Virus testing assays included: rhinovirus (RV) [26], influenza viruses (A and B) [27], respiratory syncytial viruses (A and B) [28], parainfluenza viruses (1?) [29], human adenoviruses [22], humanTable 1 Oligonucleotide primers for equine herpes virus-1 (EHV 1)
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E response genes coincided with suppressed antiviral genes, indicating the importance of investigating co-infection dynamics of salmon responding to both lice and viruses. MethodsAnimals and exposurePink and chum salmon were obtained as swim-up fry (
Phase bacterial cells were fixed in 0.08 (wt/vol) paraformaldehyde and incubated for 1 hour at 37uC. Fixed bacteria were then washed once with PBS, resuspended in Newborn Calf Serum (Sigma) and incubated for 20 min. at 25uC. The cells were then incubated for 1 hour at 4uC in presence of mAbs diluted 1:200 in dilution buffer (PBS, 20 Newborn Calf Serum, 0.1 BSA). Cells were washed in PBS-0.1 B is a Free Page Rank 5 DoFollow Social Bookmarking Site. You can submit your Stories on various Categories like Technology, Internet, Business, and lots more!

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