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E response genes coincided with suppressed antiviral genes, indicating the importance of investigating co-infection dynamics of salmon responding to both lice and viruses. MethodsAnimals and exposurePink and chum salmon were obtained as swim-up fry (
Phase bacterial cells were fixed in 0.08 (wt/vol) paraformaldehyde and incubated for 1 hour at 37uC. Fixed bacteria were then washed once with PBS, resuspended in Newborn Calf Serum (Sigma) and incubated for 20 min. at 25uC. The cells were then incubated for 1 hour at 4uC in presence of mAbs diluted 1:200 in dilution buffer (PBS, 20 Newborn Calf Serum, 0.1 BSA). Cells were washed in PBS-0.1 B
Run in duplicate, with an average intra-assay coefficient of variation of 5.2 . Samples that read as below the detection limit of 0.0001 mg/L, above 10 mg/L, or with coefficients of variation over 20 were rerun. The average intraassay coefficient of variation for the low control was 2.4 and for the high control was 3.1 . The average inter-assay coefficient of variation for the low controlwas 23
Al control contributes to the regulation of Arabidopsis photomorphogenesis. Mol. Syst. Biol. 8: 566. Lukaszewicz, M., Jerouville, B., and Boutry, M. (1998). Signs of translational regulation within the transcript leader of a plant plasma membrane H(+)-ATPase gene. Plant J. 14: 413-423. Luo, Y., and Goss, d.J. (2001). Homeostasis in mRNA initiation: wheat germ poly(A)-binding protein lowers the ac
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The latter declaration revealed that this particular phase signifies the particular overdue prediabetic cycle as well as permitted us all to perform verification from the complete selection within a few studies. All of us would not see a consistent correlation between the scale in the IFN-�� result as well as the thanks in the stimulating peptide for HLA-A0201. For instance, peptide 63 based on m
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Run in duplicate, with an average intra-assay coefficient of variation of 5.2 . Samples that read as below the detection limit of 0.0001 mg/L, above 10 mg/L, or with coefficients of variation over 20 were rerun. The average intraassay coefficient of variation for the low control was 2.4 and for the high control was 3.1 . The average inter-assay coefficient of variation for the low controlwas 23
Y centrifugation and resuspended in HBSS buffer. Briefly, the reactions took place in a total volume ofPLOS Computational Biology | www.ploscompbiol.orgEpitope mapping with monoclonal antibodiesThe epitope-mapping approach was based on the method described by Peter and Tomer [24], which we adapted to the following two protocols [25,26]:1) Immunocapturing of peptides from antigen partial digestion
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Than 0.16. Accordingly, individual scores on the first principle component were used in subsequent analyses as measures of global adiposity. We calculated an average linear height velocity for each participant by averaging the change in height between each season, adjusting for duration of time between measurements. Two data points were identified as outliers and removed; one had an associated he
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